Crispr/cas12a coupled with recombinase polymerase ampli fi cation for sensitive and speci c detection of aphelenchoides besseyi

HIGHLIGHTS

  • who: Recombinase Polymerase and collaborators from the China Normal University, China have published the research: CRISPR/Cas12a Coupled With Recombinase Polymerase Ampli fi cation for Sensitive and Speci c Detection of Aphelenchoides besseyi, in the Journal: (JOURNAL)
  • how: The results showed that 1/100 and 1/101 dilutions developed a clear positive test band implying that the detection limit of the RPA-Cas12a-Ab LFA assay was 0.96 ng/u03bcl gDNA of A. besseyi. The results showed that the assay accurately detected A. besseyi DNA without false-positive signals from other nematodes confirming the . . .

     

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