Crispr-cas12a nucleases function with structurally engineered crrnas: synthetic tracrrna

HIGHLIGHTS

• who: D. J. Jedrzejczyk from the Technical University have published the paper: CRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA, in the Journal: Scientific Reports Scientific Reports
• what: One of the major goals in cell engineering is to site-specifically insert DNA payloads via Homology-Directed Repair (HDR). The authors explored and tested CRISPR-Cas12a-based editing systems. The study showed efficient cleavage caused by AsCas12a charged with high dose of spacer RNA was capable of cleavage of DNA su00ad ubstrates18. In line with this, the authors show that the MAD7 nuclease also . . .
  

   

  

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