Crispr/cas9 editing of the myo7a gene in rhesus macaque embryos to generate a primate model of usher syndrome type 1b

HIGHLIGHTS

• who: Junghyun Ryu from the DivisionScience University have published the Article: CRISPR/Cas9 editing of the MYO7A gene in rhesus macaque embryos to generate a primate model of Usher syndrome type 1B, in the Journal: Scientific Reports Scientific Reports of 11/03/2022

SUMMARY

Four gRNAs targeting exon 3 of MYO7A were developed and initially tested for their targeting efficiency in_vitro. Cas9 protein and each hyb-gRNA were combined with PCR amplicons containing the wild-type MYO7A exon 3 target site. Three out of four hyb-gRNAs (#1 through #3) were subsequently tested . . .

 



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