Crispr/ cas9 establishment-mediated targeted mutagenesis in macrobrachium nipponense

HIGHLIGHTS

  • who: March and collaborators from the Shantou University, China have published the Article: CRISPR/ Cas9 establishment-mediated targeted mutagenesis in Macrobrachium nipponense, in the Journal: (JOURNAL)
  • how: The amplification procedure of qPCR was followed as described in previous studies with eIF gene as the internal reference gene and data were calculated by using the 2-u0394u0394CT method and SPSS 23.0 (Hu et_al 2018). The results showed that Mn-Vg expression was very low in the early embryo stages and it significantly increased after the larvae hatching stage . The results showed that three mutant sequences . . .

     

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