Crispr/cas9-mediated knock-in strategy at the rosa26 locus in cattle fetal fibroblasts

HIGHLIGHTS

  • who: Yuxuan Xie et al. from the Key Laboratory of Songliao Aquatic Environment, Ministry of Education, Jilin jianzhu University, Changchun, Jilin Province, People`s Republic of China, College of Animal Science and Technology, China have published the research: CRISPR/Cas9-mediated knock-in strategy at the Rosa26 locus in cattle fetal fibroblasts, in the Journal: PLOS ONE of 14/Oct/2022
  • what: The authors selected the intron of the cRosa26 locus for targeting and designed 3 guide RNAs (sgRNAs) (Fig 1A).

SUMMARY

    Whether this locus supports exogenous gene_expression under exogenous promoter . . .

     

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