Crispr/cas9-mediated multiplexed genome editing in aspergillus oryzae

HIGHLIGHTS

  • who: Qinghua Li and colleagues from the Science Center for Future Foods, Jiangnan University, Lihu Road, Wuxi, China have published the article: CRISPR/Cas9-Mediated Multiplexed Genome Editing in Aspergillus oryzae, in the Journal: (JOURNAL)
  • what: In this study, when yA and three copies of the amylase gene were edited, it could theoretically be considered simultaneous editing of four genes, and the editing efficiency of the knockout was 4.9%.

SUMMARY

    With the in-depth application of A. oryzae in many fields, establishing efficient gene editing technology is of great significance . . .

     

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