Crispr/cas9-mediated point mutations improve α -amylase secretion in saccharomyces cerevisiae

HIGHLIGHTS

• who: Advance access publication date and colleagues from the Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen, Gothenburg, Sweden have published the Article: CRISPR/Cas9-mediated point mutations improve α -amylase secretion in Saccharomyces cerevisiae, in the Journal: (JOURNAL) of March/17,/2022
• what: In this work, three allele replacement strategies were designed to introduce these amino_acid-altering mutations.
• how: To summarize the authors presented three CRISPR/Cas9-based strategies to introduce nucleotide substitutions in this work. The result showed that ERV29 deletion reduced the protein secretion by 67% (Fig . . .
  

   

  

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