Crispr-dcas13-tracing reveals transcriptional memory and limited mrna export in developing zebrafish embryos

HIGHLIGHTS

  • who: Youkui Huang from the State Key Laboratory University of Shanghai, China have published the research work: CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos, in the Journal: (JOURNAL)
  • what: The authors report that zygotic microinjection of purified CRISPR-dCas13-fluorescent proteins and modified guide RNAs allows single- and dual-color tracking of develu2011 opmentally expressed mRNAs in zebrafish embryos from zygotic activau2011 tion (ZGA) until early segmentation period without genetic manipulation. The authors aimed to develop a robust CRISPR-dCas13 RNA imaging system to address questions concerning the . . .

     

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