HIGHLIGHTS
- who: Subject Category Membranes Trafficking and colleagues from the Strains and plasmids Genes were deleted by replacing a complete open reading frame with a nat, (Janke et al, ) or a loxP-flanked kan, deletion cassette (Gu00fc uldener et al, )Some constructs required to remove the kanMX4, using the Cre-lox P recombination method with plasmid p, (Euroscarf #P30114). Atg, and CSC have been, terminally tagged with either Gly6-FLAG3::kan, (available on Addgene #20754) (Funakoshi and Hochstrasser, ). yomCherry:: kanMX4, and yomCherry::Sp, (both available on Addgene #, and #44841) (Lee et al, ) by direct insertion of these tags . . .
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