Cryopreservation does not change the performance and characteristics of allogenic mesenchymal stem cells highly over-expressing a cytoplasmic therapeutic transgene for cancer treatment

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SUMMARY

    The freshly modified MSCs demonstrated high anti-cancer efficacy in both in_vitro and in_vivo studies. From a regulatory perspective, cryopreservation offers assurance on the safety of the therapeutic doses by providing time for product standardisation, in_vitro assays validation and quality_control testing. Viral modified MSCs stably expressing a transgene by chemical selection has been successfully cryopreserved without affecting their transgene_expression, cell viability and tumour tropism ability of MSCs. As there is no genome integration, transgene_expression can be reduced or lost post-cryopreservation. A recent study reported that cell viability and transgene_expression of the thawed cells . . .

     

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