Crystal structure of a highly conserved enteroviral 5⠲ cloverleaf rna replication element

HIGHLIGHTS

• who: Naba K. Das from the Fab expression and purification Fab BL3, expression plasmid was a kind gift from Joseph Piccirilli, the University of ChicagoThe Fab was purified according to the published protocols52, , . Briefly, the plasmid was transformed into , E. coli competent cells and streaked into an LB-agar plate with , u00b5g/ml of carbenicillin. Several colonies were selected to inoculate a , ml starter culture and grown at , u00b0C for , h. The starter culture was then used to inoculate , liter of, xYT media, and cells were grown for , h at , u00b0C. For Fab overexpression, the cells were . . .
  

   

  

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