Cyclin f/fbxo1 interacts with hiv-1 viral infectivity factor (vif) and restricts progeny virion infectivity by ubiquitination and proteasomal degradation of vif protein through scfcyclin f e3 ligase machinery*

HIGHLIGHTS

• who: from the cal Institute Blood Bank, Navi Peth, Pune, India, and PBMCs were isolated using Histopaque , (Sigma) by gradient centrifugationCD4⫹ T cells were purified from PBMCs using flow cytometry-based cell sorting. Jurkat and HEK T cell lines were obtained from the NCCS Cell Repository, Pune, India. CEM-GFP (catalog No., ) (60) and TZM-bl (catalog No., ) (61) were obtained from the NIH AIDS Reagent Program, Division of AIDS, NIAID, National Institutes of Health. HEK T and TZM-bl cells were grown in DMEM (Invitrogen), and PBMCs, purified CD4⫹ T cells, and Jurkat and CEMGFP cells . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.