Cytochrome p450cin (cyp176a), isolation, expression, and characterization*

HIGHLIGHTS

• who: David B. Hawkes from the Materials-Yeast extract and Bacto-tryptone were obtained from BD PharMingenReagent grade, cineole was obtained from Aldrich. Sodium dithionite (sodium hydrosulfite) was purchased from Aldrich. Nylon hybridization transfer membranes (GeneScreen Plus) were obtained from PerkinElmer Life Sciences. All other chemicals used were purchased from commercial sources and were of the highest grade generally available. The restriction enzymes and polynucleotide kinase were obtained from New England Biolabs, Inc. (Beverly, MA), and Roche Molecular Biochemicals based on availability. Terminal deoxynucleotidyltransferase was obtained from Promega Corp. (Madison, WI). Escherichia coli strain, DH5u2423Fu2b18 u243e80dlacZu232c, u232c . . .
  

   

  

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