Data-independent acquisition boosts quantitative metaproteomics for deep characterization of gut microbiota

HIGHLIGHTS

  • who: Jinzhi Zhao from the Published in partnership with Nanyang Technological University have published the paper: Data-independent acquisition boosts quantitative metaproteomics for deep characterization of gut microbiota, in the Journal: (JOURNAL) of 11/01/2023
  • what: The authors demonstrated superior performance of directDIA based on a comprehensive consideration of proteome coverage, as well as accuracy and precision in quantification. Ratio compression limited TMT quantification accuracy for the spike-in metaproteome samples, and directDIA outperformed TMT in quantitative analysis of the spike-in metaproteome samples, even though MS3-based quantification was used in this study . . .

     

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