De novo determination of mosquitocidal cry11aa and cry11ba structures from naturally-occurring nanocrystals

HIGHLIGHTS

• who: Guillaume Tetreau from the cry11ba geneThe list of primers used for plasmids construction is available in Supplementary Table, . For each plasmid construction, the fragments with overlapping overhangs were assembled using the NEBuilder HiFi DNA Assembly (New England have published the research work: De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
• what: As the crystals are naturally labile at pH 11, the authors aimed at collecting data from crystals soaked at slightly lower pH than 11, hypothesizing that early conformational changes would show . . .
  

   

  

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