HIGHLIGHTS
SUMMARY
The use of internal controls when performing quantitative gene_expression analysis (such as microarrays, RNA-sequencing, and quantitative reverse_transcriptase-polymerase_chain_reaction ) represents the most common strategy to normalize gene_expression to correct for intrinsic errors related to sample manipulation and the technical protocol. Given their fundamental roles, HKGs tend to display medium-high expression levels; this characteristic makes these genes especially suitable as internal controls/reference genes to normalize gene_expression data in quantitative gene_expression analysis. Meanwhile, Oliva et_al reported the impact of sex on gene_expression in various human tissues through metadata_analysis by the GTEx platform, generating a . . .
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