Deep learning driven adaptive optics for single molecule localization microscopy

HIGHLIGHTS

  • who: fangh from the School of Biomedical Engineering, University, West Lafayette, IN, USA have published the research: Deep Learning Driven Adaptive Optics for Single Molecule Localization Microscopy, in the Journal: (JOURNAL)
  • what: The authors demonstrate DL-AO`s capacity in compensating significant index mismatch induced aberrations using constructed specimens from imaging media.
  • how: To characterize DL-AO`s capacity for PSF restoration the authors introduced random wavefront distortions using the deformable mirror and compensated these distortions with DL-AO during SMLM experiments with immune- fluorescence-labeled TOM20 in COS-7 cells.
 

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