Deep proteome coverage of microglia using a streamlined data-independent acquisition-based proteomic workflow: method consideration for a phenotypically diverse cell type

HIGHLIGHTS

• What: This study implemented a streamlined liquid- and gas-phase fractionation method with data-dependent acquisition (DDA) and parallel accumulation-serial fragmentation (PASEF) analysis on a TIMS-TOF instrument to compile a comprehensive protein library obtained from adult-derived immortalized mouse microglia with low starting material (10 µg). The authors report a simplified reproducible approach to address the proteome complexity of microglia using low sample input and show the importance of library optimization for this phenotypically diverse cell type. The authors evaluated the potential complementarity of the IMG-derived DIA output using both the empirical and predicted . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.