Deletion of the fih gene encoding an inhibitor of hypoxia-inducible factors increases hypoxia tolerance in zebrafish

HIGHLIGHTS

• who: Xiaolian Cai from the the Institute of Hydrobiology, Chinese Academy of Sciences (Wuhan, Hubei, China)We injected ⬃, one-cell embryos with the indicated plasmids: HRE reporter (, pg/embryo), Myc-empty (, pg/embryo), Myc-fih (, pg/embryo), Myc-hif1␣a (, pg/embryo), Myc-hif1␣b (, pg/embryo), Myc-hif2␣a (, pg/embryo), and Myc-hif2␣b (, pg/embryo). At , h postfertilization (hpf), embryos from each group were harvested and homogenized. We measured luciferase activity in these embryos using the Dual-Luciferase reporter assay system (Promega). Luciferase activity was normalized to Renilla luciferase. Western blotting have published the . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or E-mail:

  If you don't have an account, you can create one here.