Deletion of the transmembrane protein prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration

HIGHLIGHTS

• who: Zhaojing Lu from the zebrafish were approved by the Ethics Committee of Huazhong University of Science and Technology and were in accordance with Animal Research: Reporting of in Vivo Experiments (ARRIVE) guidelinesCell culture and transfection Cells were cultured in Dulbecco's modified Eagle's medium (human lymphatic endothelial cells and HeLa) or Dulbecco's modified Eagle's medium/, medium (ARPE-19) containing, % fetal bovine serum and incubated at , °C in, % , in a humidified incubator. When grown to , % confluence, cells were replated at a split ratio of, :3-1:5. Cell transfection was performed with LipofectamineTM , (Invitrogen . . .
  

   

  

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