Denaturing gradient gel electrophoresis approach for microbial shift analysis in thermophilic and mesophilic anaerobic digestions

HIGHLIGHTS

• What: To determine the evolution of microbial community and microbial shift under anaerobic processes this study investigates the use of electrophoresis (DGGE). At day 60 the analysis showed that DNA fragments of effluent of 28 ◦ C reactor were similar to Galbibacter mesophilus (strain: NBRC 10162) (similarity: 87%) and DNA fragments of effluent of 36 ◦ C reactors were similar to Syntrophomonas curvata (strain: GB8-1) (similarity: 91%). The use of the DGGE gel approach is explored previously on processed waste to target typical bacteria of stabilized organic waste, and the approach was able to provide distinctive microbial fingerprints . . .
  

   

  

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