Derepression of the usp22-fasn axis by p53 loss under oxidative stress drives lipogenesis and tumorigenesis

HIGHLIGHTS

• who: Zelong Han from the Plasmid construction Flag-FASN plasmid was kindly provided by DrDan Ye (Fudan University). The cDNA of FASN truncation mutants was amplified from Flag-FASN by PCR and cloned into pcDNA3.1-Flag vector. HA-, and other DUBs were kept in the lab. Full length cDNAs of human FASN and , were also subcloned into the pLVX-neo vector. Plasmids expressing , shRNAs were constructed by annealing the sense and antisense oligonucleotides of the target sequence and cloned into the pLKO.1-puro vector. All plasmids were confirmed by DNA sequencing. Table , contains detailed information . . .
  

   

  

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