Design and directed evolution of noncanonical β-stereoselective metalloglycosidases

HIGHLIGHTS

  • who: Woo Jae Jeong from the Department of Chemistry, Seoul National University, Seoul, Republic of Korea have published the article: Design and directed evolution of noncanonical u03b2-stereoselective metalloglycosidases, in the Journal: (JOURNAL) of 29/Nov/2021
  • what: The authors design metalloglycosidases by constructing a hydrolytically active Zn-binding_site within a barrel-shaped outer membrane protein OmpF. This work shows that unprecedented metalloenzymes can be tailor-made, expanding the scope of inorganic reactivities in proteinaceous environments, resetting the structural and functional diversity of metalloenzymes, and providing the potential molecular basis of unidentified metallohydrolases and novel . . .

     

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