Design and experimental validation of opera_met-a panel for deep methylation analysis by next generation sequencing

HIGHLIGHTS

  • who: Federico Pio Fabrizio et al. from the Harvard Medical School, United States University of Turin, Italy have published the research work: Design and experimental validation of OPERA_MET-A panel for deep methylation analysis by next generation sequencing, in the Journal: (JOURNAL)
  • what: In this methodology paper, the authors designed and experimentally validated a customized methylation panel for NGS analysis (OPERA_MET-A panel), to scan relevant CpG sites in 155 regions of 18 cancer-related genes mainly involved in the NRF2/KEAP1 pathway and immunotherapy. gDNA from FFPE, frozen tissues and cell lines were tested . . .

     

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