Detection of klebsiella pneumonia dna and esbl positive strains by pcr-based crispr-lbcas12a system

HIGHLIGHTS

  • who: ufeffShangufeff ufeffWangufeff from the Zhejiang University, China have published the research work: Detection of ufeffKlebsiella pneumoniaufeff DNA and ESBL positive strains by PCR-based CRISPR-LbCas12a system, in the Journal: (JOURNAL) of 09/02/2023
  • what: The authors speculate that this contradiction may result from the following reasons:
  • how: The authors compared PCR-LbCas12 workflow with PCR and qPCR techniques. Establishment of PCR-LbCas12a detection targeting Klebsiella pneumonia To find a suitable target for nucleic_acid detection the authors used blast to screen the most relevant gene as a target for nucleic_acid detection . . .

     

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