Detection of functional protein domains by unbiased genome-wide forward genetic screening

HIGHLIGHTS

  • who: Mareike Herzog from the (UNIVERSITY) have published the Article: Detection of functional protein domains by unbiased genome-wide forward genetic screening, in the Journal: (JOURNAL) of 22/03/2018
  • what: The authors show that olaparib resistance is linked to missense mutations in the DNA binding regions of PARP1, but not in its catalytic domain.

SUMMARY

    In contrast to gene disruption/depletion strategies to identify mechanisms of drug resistance, searching for point-mutational genetic suppressors that can identify separation- or gain-of-function mutations has been limited. By demonstrating its utility . . .

     

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