HIGHLIGHTS
- who: Benoit J. Pons from the Environment and Sustainability Institute, Biosciences, University of Exeter, Penryn, UK have published the research work: Determination of Acr-mediated immunosuppression in Pseudomonas aeruginosa, in the Journal: (JOURNAL)
- how: For the Pseudomonas P. aeruginosa PA14 strain the authors used a protospacer matching spacer 1 of CRISPR array 2 of the type I-F CRISPR-Cas system . For the SMC4386 protospacer tests the authors used 12 mL of overnight culture. u25e6 Transfer overnight culture in 1.5 mL microcentrifuge tubes (2 tubes for the non-targeted plasmid and 2 tubes per . . .
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