Development and application of a rapid all-in-one plasmid crispr-cas9 system for iterative genome editing in bacillus subtilis

HIGHLIGHTS

• who: Yu Zou from the State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, People’s Republic of China have published the research work: Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis, in the Journal: (JOURNAL) of 14/08/2022
• what: We developed and applied a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in B. subtilis by constructing ­PacoR-sgRNArep self-curing module.
• how: At last as a proof . . .
  

   

  

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