Development of a high-sensitivity and short-duration fluorescence in situ hybridization method for viral mrna detection in hek 293t cells

HIGHLIGHTS

• who: Dailun Hu from the University of Su00e3o Paulo, Brazil have published the article: Development of a high-sensitivity and short-duration fluorescence in situ hybridization method for viral mRNA detection in HEK 293T cells, in the Journal: (JOURNAL)
• what: The authors have only compared the method with other methods from methodological viewpoints.
• how: For experiments with a 2-h hybridization time the authors utilized 800 ng probe (400 ng Probe 1 + 400 ng Probe 2) in 40 ml hybridization buffer. As a positive control for the FISH method an Oligo dT probe . . .
  

   

  

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