Development of a new taqman-based real-time rt-pcr assay for the specific detection of bovine kobuvirus

HIGHLIGHTS

• who: Ruiwen Li and Jianchang Wang from the The University of Sydney, Australia have published the Article: Development of a new TaqMan-based real-time RT-PCR assay for the specific detection of bovine kobuvirus, in the Journal: (JOURNAL)
• what: The authors designed specific primers and TaqMan probe based on the 3D gene of BKV and developed a real-time RT-PCR assay for BKV. The analysis was repeated three times and the PCR amplification efficiency (E), standard curve slope, and correlation coefficient (R2 ) were calculated as described previously .
• how: All the extracted . . .
  

   

  

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