Differential effects of mutations of popdc proteins on heteromeric interaction and membrane trafficking

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• who: Alexander H. Swan from the Full length human , and , cDNAs were inserted into the pECFP-N1/pEYFP-, plasmid, to append a, terminus ECFP/EYFP tagThe clinically identified POPDC variants, and the aspartic acid scanning mutations of hydrophobic residues in the u03b1C-helix, were introduced into these constructs using the , sitedirected mutagenesis kit (NEB). The oligonucleotide primer sequences used for the site-directed mutagenesis are listed in Additional file, : Table, . For NanoBRET analysis, human , and , cDNA sequences were cloned into the pFC K or pFC K plasmids (Promega), which contain, terminus sequences for HaloTag and NanoLuc tags . . .
  

   

  

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