Differential grainy head binding correlates with variation in chromatin structure and gene expression in

HIGHLIGHTS

• who: Henry A. Ertl from the , lightly fixed imaginal wing discs were used for each sampleFor CutandRun [20], the protocol provided with the Cell Signaling Cut and Run Kit (CAT: S) was used with the following minor modifications and specifications: (1), uL (instead of , mL) of, x Wash Buffer was used to dounce homogenize the wing discs to ensure efficient pelleting, (2) the provided spike-in DNA was added at, :, dilution, and (3) for each sample, we used, uL of a Grainy head antibody that targets an epitope on the, terminus of Drosophila Grh [21]. To construct . . .
  

   

  

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