Differential placental dna methylation of nr3c1 in extremely preterm infants with poorer neurological functioning

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  • who: Nienke H. van Dokkum from the The University of Queensland, Australia have published the Article: Differential Placental DNA Methylation of NR3C1 in Extremely Preterm Infants With Poorer Neurological Functioning, in the Journal: (JOURNAL)
  • how: For polymerase_chain_reaction (PCR) amplification the authors used a master mix of 12.5 u00b5l HotStarTaq DNA Polymerase 10.5 u00b5l sterile water a 1 u00b5l mix of forward and reverse primer and a 1 u00b5l bisulfite template according to the manufacturer`s instructions. Information on the pyrosequencing method used and two representative examples of the sequencing results are provided in . . .

     

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