Diffusional spread and confinement of newly exocytosed synaptic vesicle proteins

HIGHLIGHTS

• who: Niclas Gimber from the mice (P0-P3) were transfected with pFUGW-based plasmids, co-expressing mKate and specific shRNA directed against , to obtain AP180/CALM-depleted neuronsAs a control, hippocampal neurons from WT mice (P0-P3) co-expressing mKate and scrambled control shRNA were used. Plasmids and antibodies. Plasmids encoding for Syb, (WT, rat) and Syt, (rat) with a TEV protease cleavable superecliptic pHluorin-tag were a kind gift from J. Klingauf (University of Münster, Münster, Germany)7. Mutant Syb, (M A) was cloned into a pHluorin plasmid by PCR amplification and standard cloning . . .
  

   

  

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