Digital droplet pcr analysis of organoids generated from mouse mammary tumors demonstrates proof-of-concept capture of tumor heterogeneity

HIGHLIGHTS

• What: The authors show it is technically feasible to evaluate copy number (CN) heterogeneity of tumors and organoids at various resolutions, including pooled organoid samples, single organoids, and invading organoids, using clinically relevant target genes. While these sites of metastasis have distinct biology, the purpose of this study aims to provide a framework to study how genetic heterogeneity at a primary tumor site may be reflected in the metastatic process. The authors assessed organoid CNAs without passaging, however organoid CNAs could evolve over time through longterm culture and passaging, as previously described (Sachs et_al, 2018). The authors . . .
  

   

  

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