Direct correction of haemoglobin e β-thalassaemia using base editors

HIGHLIGHTS

• who: Mohsin Badat from the , + cells were differentiated down the erythroid lineage using a modification of a published differentiation protocol All phases used a prepared base media containing Iscove`s modified Dulbecco`s media (Bioscience UK), u00b5g/ml human holo-transferrin (HT) (RandD systems), u00b5g/ml recombinant human insulin (Sigma Aldrich), IU/ml heparin sodium (Sigma Aldrich), % inactivated group AB Plasma (Department of Haematology, Oxford University Hospitals Trust), IU/ ml erythropoietin (Janssen-Cilag) and, % foetal bovine serum. Phase , (Day , to, ), Freshly isolated or thawed HSPCs were seeded at a density of , u00d7 , cells/ml in base media . . .
  

   

  

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