Direct determination of multiple ligand interactions with the extracellular domain of the calcium-sensing receptor*

HIGHLIGHTS

  • who: Chen Zhang from the (UNIVERSITY) have published the research work: Direct Determination of Multiple Ligand Interactions with the Extracellular Domain of the Calcium-sensing Receptor*, in the Journal: (JOURNAL) of November/28,/2014
  • how: The 1H-15NHSQC data were analyzed by Sparky and the chemical shift perturbations were calculated using the equation ⌬ ␦ av ⫽ 冑0.5 ⫻ 关共⌬␦1H兲2 ⫹ 0.07 ⫻ 共⌬␦15N兲2兴 (Eq 4) where ⌬␦av is the chemical shift perturbations of the 1H-15N spectra ⌬␦ is the chemical shifts for 1H or15N . The secreted recombinant CaSR ECD containing complex glycosylation is able to bind . . .

     

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