Direct digital sensing of protein biomarkers in solution

HIGHLIGHTS

• who: Georg Krainer from the Protein preparation The monovalent streptavidin sample was kindly provided by the Howarth Lab (University of Oxford)23, and recombinant IgE Kappa (clone AbD18705_IgE, Cat#HCA190) was purchased from Bio-Rad LaboratoriesWild-type u03b1-synuclein was recombinantly expressed and purified according to published procedures84, . Briefly, Escherichia coli BL21(DE3) cells were transformed with a pT7, expression vector encoding the gene for human wild-type u03b1-synuclein. Cultured cells were grown at , u00b0C under shaking in Luria Bertani medium supplemented with Ampicillin (, u03bcg/mL) to an optical density at , nm of, ., Expression was induced . . .
  

   

  

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