Direct in situ protein tagging in chlamydomonas reinhardtii utilizing tim, a method for crispr/cas9-based targeted insertional mutagenesis

HIGHLIGHTS

• who: Yuqing Hou and collaborators from the Division of Cell Biology and Imaging, Department of Radiology, University of Massachusetts Chan Medical have published the article: Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis, in the Journal: PLOS ONE of 18/07/2022
• what: The authors develop a related strategy, based on TIM, that enables direct, highly efficient tagging of specific C. reinhardtii genes in situ. The authors demonstrate the feasibility of this new strategy by successfully tagging LF5/CDKL5, a protein involved in flagellar . . .
  

   

  

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