HIGHLIGHTS
- who: Fien Gysens from the Department Ghent University have published the Article: Direct lysis of 3D cell cultures for RT-qPCR gene expression quantification, in the Journal: Scientific Reports Scientific Reports
- how: For spheroid lysis the authors used 8 or 16 spheroids. For the biologically relevant genes the authors used the following primers IL8 (Bio-Rad primer qHsaCED0046633) NFkB (Bio-Rad primer qHsaCED0002379) NEAT1 (FWD GGAu200bGAGu200bGGTu200bTGGu200bTTAu200bGAGu200bAT REV CCTu200bTCAu200bACCu200bTGCu200bATTu200bTCCu200b TA) MALAT1 (FWD AGTu200bTCGu200bTGGu200bTGAu200bAGAu200bTAGu200bGA REV TAGu200bCTTu200bCCTu200bTCAu200bCCAu200bAATu200bCG) and TGFBR1 (FWD TCAu200bGCTu200bCTGu200bGTTu200bGGTu200bGTCu200bAG REV ATGu200bTGAu200bAGAu200bTGGu200bGCAu200bAGAu200bCC).
SUMMARY
The authors used two different respiratory epithelial_cell lines, A549 and . . .
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