Direct pcr with the cdc 2019 sars-cov-2 assay: optimization for limited-resource settings

HIGHLIGHTS

  • who: Christia M. Victoriano from the DepartmentVanderbilt University Medical Center, Nashville, TN, USA have published the research: Direct PCR with the CDC 2019 SARS-CoV-2 assay: optimization for limited-resource settings, in the Journal: Scientific Reports Scientific Reports
  • what: The authors compared standard clinical-use CDC procedures to the direct pipetting of heat-inactivated viral transport medium (VTM) sample into the qRT-PCR reaction. This approach is easily scalable because large batches of tubes can be simultaneously heat-treated and more patient specimens can be analyzed on a single qPCR plate.
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