Direct reprogramming of human fibroblasts into insulin-producing cells using transcription factors

HIGHLIGHTS

• who: Marta Fontcuberta-PiSunyer from the detached from the mold and maintained in tissue culture dishes with warm , medium until transplant (usually, h)Constructs were placed on the omentum close to the duodenal-stomach junction. Alternatively, constructs were introduced in the abdominal subcutaneous space through a small (, mm) incision. Transplantation in the kidney was performed following standard procedures79. NSG mice transplanted with fibroblast spheroids an/or non-transplanted NSG mice were used as controls in transplantation experiments. To assess vascularization and cell viability in ACE implants, TF cell spheroids were labeled with the long-term tracer for . . .
  

   

  

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