Directed evolution of cole1 plasmid replication compatibility: a fast tractable tunable model for investigating biological orthogonality

HIGHLIGHTS

  • who: Santiago Chaillou and collaborators from the KU Leuven, Department of Pharmaceutical and Pharmacological Sciences, Rega Institute for Medical Research, Herestraat, Belgium and , University College London, Institute of Structural and Molecular Biology, University College London, Gower Street, London WC E BT, UK have published the Article: Directed evolution of colE1 plasmid replication compatibility: a fast tractable tunable model for investigating biological orthogonality, in the Journal: (JOURNAL)
  • what: The authors show that by diversifying loop regions in RNAI (and RNAII) it is possible to select new viable colE1 origins compatible with the wildtype one. The authors . . .

     

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