Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform

HIGHLIGHTS

• who: Linyue Zhang from the (UNIVERSITY) have published the article: Directed evolution of phosphite dehydrogenase to cycle noncanonical redox cofactors via universal growth selection platform, in the Journal: (JOURNAL) of 11/02/2022
• what: Using this system, the authors develop a phosphite dehydrogenase (PTDH) to cycle NMN+ with ~147-fold improved catalytic efficiency, which translates to an industrially viable total turnover_number of ~45,000 in cell-free biotransformation without requiring high cofactor concentrations.
• how: Media and growth conditions Bacterial strains and plasmids used in this work are described in the Supplementary Data 1 . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.