Discovery of streptococcus pneumoniae serotype 6 variants with glycosyltransferases synthesizing two differing repeating units*

HIGHLIGHTS

• who: Melissa B. Oliver from the cell debris by centrifugation, PS was precipitated stepwise in, and, % ethanol and was recovered by dissolution in , mM NaClAfter desalting by dialysis against MilliQ water, the PS was loaded onto a, ml column of DEAE-Sepharose (GE Healthcare) and eluted with a linear gradient of NaCl from , to , M. The resulting fractions were tested for PS using a multibead inhibition assay (22). The PS-containing fractions were pooled, desalted by dialysis, lyophilized, dissolved in, ml of water, and loaded onto a gel filtration column (, ml of Sephacryl , HR have published the . . .
  

   

  

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