Disease-linked mutations in the phosphatidylcholine regulatory enzyme cctα impair enzymatic activity and fold stability

HIGHLIGHTS

• who: Rosemary B. Cornell from the Preparation of mutant forms of human PCYT A pET14b plasmids containing human PCYT A and the lipodystrophy-related PCYT A alleles were generously donated by Iona Isaac and David Savage, Cambridge University, UKExpression in Escherichia coli was very poor due to proteolysis by endogenous bacterial proteases. The cDNAs were transferred into the COS-cell expression vector p, by first engineering MluI and SalI restriction sites flanking the, ⬘ and, ⬘ ends of the cDNAs, and were then cut and inserted into the MluI/SalI sites in the p, vector. The SMD-CRD and . . .
  

   

  

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