Distinct activation mechanisms of nf-κb regulator inhibitor of nf-κb kinase (ikk) by isoforms of the cell death regulator cellular flice-like inhibitory protein (cflip)*

HIGHLIGHTS

• who: Mehdi Baratchian from the man Coulter)Viral pellets were resuspended in cold whole medium, incubated on ice for, h, aliquoted, and stored at ⫺, °C. Titers were measured on HEK T cells by fluorescence-activated cell sorting (FACS) analysis and TaqMan quantitative PCR (Applied Biosystems, Warrington, UK). For experiments, cells were transduced with test cFLIP, vFLIP, and Tax lentivectors at identical multiplicities of infection within experiments and in the range of, or , for, T or Z/3, respectively. Generation of Stable Knock-down Cell Lines-shRNA-expressing lentivectors targeting HOIP, TAK1, Atg3, caspase-8, RIP1, and FADD . . .
  

   

  

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