Distinct domains of the ù²1-subunit cytosolic n terminus control surface expression and functional properties of large-conductance calcium-activated potassium (bk) channels

HIGHLIGHTS

• who: Lie Chen from the Expression constructs Plasmids encoding the full-length BK channel ␣-subunit ZERO splice variant with either an, terminal FLAG tag or both a FLAG- and a, terminal HA (-HA) tag were described previously (26)A sticky-end PCR cloning strategy was adopted to generate, mycc-tagged ␤1-subunit constructs in the pcDNA3., vector. The human ␤1-subunit was first amplified from a construct (a generous gift from Dr. Jon Lippiat, University of Leeds have published the research: Distinct domains of the Ù²1-subunit cytosolic N terminus control surface expression and functional properties of . . .
  

   

  

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