HIGHLIGHTS
SUMMARY
Immunoassays are ubiquitous bioanalytical techniques, in which the presence of a target molecule is detected or quantified using antibody-antigen binding interaction.[1] Despite tremendous importance in both fundamental research and real-world applications ranging from antibody discovery and analyte detection to medical diagnosis,[2] their development remains time-consuming, costly and require dedicated human and instrumental resources. By co-encapsulating coding DNA, expression machinery, a capture scaffold and EGFP antigen in microfluidic-generated picoliter drops, the authors assessed the antibody-antigen interaction along the course of expression (a few hours) inside individual drops . . .
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