Dramatic broadening of the substrate profile of the aeromonas hydrophila cpha metallo-β-lactamase by site-directed mutagenesis*

HIGHLIGHTS

• who: Carine Bebrone from the (UNIVERSITY) have published the article: Dramatic Broadening of the Substrate Profile of the Aeromonas hydrophila CphA Metallo-β-lactamase by Site-directed Mutagenesis*, in the Journal: (JOURNAL) of December/14,/2004
• what: The authors generated site-directed mutants that possess a strongly broadened activity spectrum when compared with the WT CphA.
• how: When binding of the second zinc ion resulted in a complete loss of activity the data were analyzed Equation 1 k cat/kcat(0) ⫽ ⫹ KD2)] (Eq 1) where kcat/kcat(0) is the ratio between kcat in . . .
  

   

  

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